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axioobserver d1 fluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axioobserver d1 fluorescence microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Axioobserver D1 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioobserver d1 fluorescence microscope/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    axioobserver d1 fluorescence microscope - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity"

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    Journal: bioRxiv

    doi: 10.1101/2025.07.11.664221

    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Figure Legend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Techniques Used: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison